平成26年-平成30年 文部科学省科学研究費補助金新学術領域研究「細胞競合」


藤田班 研究分担者(伊藤)

Tsujita, K., Takenawa, T., Itoh, T.
Feedback regulation between plasma membrane tension and membrane-bending proteins organizes cell polarity during leading edge formation.
Nat. Cell Biol. 17(6):749-758 (2015)

Tokuda, E., Itoh, T., Hasegawa, J., Ijuin, T., Takeuchi, Y., Irino, Y., Fukumoto, M., Takenawa, T.
Phosphatidylinositol 4-phosphate in the Golgi apparatus regulates cell-cell adhesion and invasive cell migration in human breast cancer.
Cancer Res. 74(11), 3054-3066 (2014)


*Nakamura M, *Ohsawa S, Igaki
T Mitochondrial defects trigger proliferation of neighbouring cells via a senescence-associated secretory phenotype in Drosophila. (*equal contribution)
Nature Communications 5: 5264, (2014)

*Takino K, *Ohsawa S, Igaki T
Loss of Rab5 drives non-autonomous cell proliferation through TNF and Ras signaling in Drosophila. (*equal contribution)
Developmental Biology 395 (1): 19-28, (2014)

Ohsawa S, Takemoto D, Igaki T
Dissecting tumor heterogeneity in flies: genetic basis of interclonal oncogenic coorperation.
J. Biochem. 156 (3): 129-36, (2014)

Igaki T, Miura M
The Drosophila TNF ortholong Eiger: Emerging physiological roles and evolution of the TNF system.
Semin Immunol. S1044-5323 (2014)

Kunimasa, Ohsawa S, Igaki
T Cell Competition: The Struggle for Existence in Multicellular Communities.
New Principles in Developmental Processes pp27-40 (2014)


Tanaka-Okamoto M, Itoh Y, Miyoshi J, Mizoguchi A, Mizutani K, Takai Y, Inoue M:
Genetic ablation of afadin causes mislocalization and deformation of Paneth cells in the mouse small intestinal epithelium.
PLoS One e110549, 2014

Suzuki T, Mizutani K, Minami A, Nobutani K, Kurita S, Nagino M, Shimono Y, Takai Y: Suppression of TGF-β1-induced protein expression of SNAI1 and N-cadherin by miR-199a.
Genes Cells 19:667-675, 2014

Yoshida T, Iwata T, Takai Y, Birchmeier W, Yamato M, Okano T:
Afadin requirement for cytokine expressions in keratinocytes during chemically induced inflammation in mice.
Genes Cells 19:842-852, 2014

Kobayashi R, Kurita S, Miyata M, Maruo T, Mandai K, Rikitake Y, Takai Y: s-Afadin binds more preferentially to the cell adhesion molecules nectins than l-afadin.
Genes Cells 19:853-863, 2014

Zhu Q, Yamakuchi M, Ture S, de la Luz Garcia-Hernandez M, Ko KA, Modjeski KL, LoMonaco MB, Johnson AD, O'Donnell CJ, Takai Y, Morrell CN, Lowenstein CJ:
Syntaxin-binding protein STXBP5 inhibits endothelial exocytosis and promotes platelet secretion.
J. Clin. Invest. 124:4503-4516, 2014

Ye S, Huang Y, Joshi S, Zhang J, Yang F, Zhang G, Smyth SS, Li Z, Takai Y, Whiteheart SW: Platelet secretion and hemostasis require syntaxin-binding protein STXBP5.
J. Clin. Invest. 124:4517-4528, 2014

Yoshida T, Takai Y, Thesleff I:
Cooperation of nectin-1 and nectin-3 is required for maintenance of epidermal stratification and proper hair shaft formation in the mouse.
Dev. Biol. J. Article ID 432043, 2014

Nakata H, Wakayama T, Takai Y, Iseki S:
Quantitative analysis of the cellular composition in seminiferous tubules in normal and genetically modified infertile mice.
J. Histochem. Cytochem. in press.

Fujiwara Y, Goda N, Tamashiro T, Narita H, Satomura K, Tenno T, Nakagawa A, Oda M, Suzuki M, Sakisaka T, Takai Y, Hiroaki H: Crystal structure of afadin PDZ domain-nectin-3 complex shows the structural plasticity of the ligand-binding site.<
Protein Sci. in press.

Mori M, Rikitake Y, Mandai K, Takai Y: Roles of nectins and nectin-like molecules in the nervous system.
Adv. Neurobiol. 8:91-116, 2014

Mandai K, Rikitake Y, Mori M, Takai Y: Nectins and nectin-like molecules in development and disease.
Curr. Top. Dev. Biol. in press.


Maruyama, T., Araki, T., Kawarazaki, Y., Naguro, I., Heynen, S., Aza-Blanc, P., Ronai, Z., Matsuzawa, A. and Ichijo, H. Roquin-2 promotes ubiquitin-mediated degradation of ASK1 to regulate stress responses.
Sci. Signal., 7, ra8 (2014).

Mosallanejad, K., Sekine, Y., Ishikura-Kinoshita, S., Kumagai, K., Nagano, T., Matsuzawa, A., Takeda, K., Naguro, I. and Ichijo, H. The DEAH-Box RNA Helicase DHX15 activates NF-κB and MAPK signaling downstream of MAVS during antiviral responses.
Sci. Signal., 7, ra40 (2014). 

Eaton, G.J., Zhang, Q.S., Diallo, C., Matuszawa, A., Ichijo, H., Steinbeck, M.J. and Freeman, T.A. Inhibition of Apoptosis Signal-Regulating Kinase 1 Enhances Endochondral Bone Formation by Increasing Chondrocyte Survival.
Cell Death & Dis., 5, e1522 (2014).

Okada, M., Matsuzawa, A., Yoshimura, A. and Ichijo, H. Lysosome rupture-activated TAK1-JNK pathway regulates NLRP3 inflammasome activation.
J. Biol. Chem., 289, 32926-32936 (2014).

Matsui, H., Fukuno, N., Kanda, Y., Kantoh, Y., Chida, T., Nagaura, Y., Suzuki, O., Nishitoh, H., Takeda, K., Ichijo, H., Sawada, Y., Sasaki, K., Kobayashi, T. and Tamura, S. The expression of Fn14 via mechanical stress-activated JNK contributes to apoptosis induction in osteoblasts.
J. Biol. Chem., 289, 6438-6450 (2014).

Noguchi, H., Yamada, S., Nabeshima, A., Guo, X., Tanimoto, A., Wang, K.Y., Kitada, S., Tasaki, T., Takama, T., Shimajiri, S., Horlad, H., Komohara, Y., Izumi, H., Kohno, K., Ichijo, H. and Sasaguri, Y. Depletion of apoptosis signal-regulating kinase 1 prevents bile duct ligation-induced necroinflammation and subsequent peribiliary fibrosis.
Am. J. Pathol., 184, 644-661 (2014).

Toyama, K., Koibuchi, N., Uekawa, K., Hasegawa, Y., Kataoka, K., Katayama, T., Sueta, D., Jie Ma, M., Nakagawa, T., Yasuda, O., Tomimoto, H., Ichijo, H., Ogawa, H. and Kim-Mitsuyama, S. Apoptosis Signal-regulating Kinase 1 is a novel target molecule for cognitive impairment induced by chronic cerebral hypoperfusion.
Arterioscler. Thromb. Vasc. Biol., 34,616-625 (2014).

Mizukami, J. Sato, T., Camps, M., Ji, H., Rueckle, T., Swinnen, D., Tsuboi, R., Takeda, K. and  Ichijo, H. ASK1 promotes the contact hypersensitivity response through IL-17 production.
Sci. Rep., 4, 4714 (2014).

Toyama, K., Koibuchi, N., Hasegawa, Y., Uekawa, K., Yasuda, O., Sueta, D., Nakagawa, T., e Ma, M., Kusaka, H., Lin, B., Ogawa, H., Ichijo, H. and Kim-Mitsuyamam S. ASK1 is involved in cognitive impairment caused by long-term high-fat diet feeding in mice.
Sci. Rep., in press.

Watanabe, T., Sekine, S., Naguro, I., Sekine, Y. and Ichijo, H. Apoptosis Signal-regulating Kinase 1(ASK1)-p38 pathway-dependent cytoplasmic translocation of the orphan nuclear receptor NR4A2 is required for oxidative stress-induced necrosis.
J. Biol. Chem., 290, 10791-10803 (2015).

Tristan, C.A., Ramos, A., Shahani, N., Emiliani, F.E., Nakajima, H., Noeh, C.C., Kato, Y., Takeuchi, T., Noguchi, T., Kadowaki, H., Sedlak, T.W., Ishizuka, K., Ichijo, H. and Sawa, A. Role of Apoptosis Signal-regulating Kinase 1 (ASK1) as an activator of the GAPDH-Siah1 Stress-Signaling Cascade.
J. Biol. Chem., 290, 56-64 (2015).

Lee, K.-W., Woo, J.-M., Im, J.-Y., Park, E.-S., He, L., Ichijo, H., Junn, E. and Mouradian M. M. Apoptosis Signal Regulating Kinase 1 Modulates the Phenotype of α-Synuclein Transgenic Mice.
Neurobiol. Aging, 36,519-526 (2015).

Zhai, J., Zhang, L., Mojsilovic-Petrovic, J., Jian, X., Thomas, J., Homma, K., Schmitz, A., Famulok, M., Ichijo, H., Argon, Y., Randazzo, P., and Kalb, R., Inhibition of cytohesins protects against genetic models of motor neuron disease.
J. Neurosci., 35, 9086-9105 (2015).

Miyakawa, K., Matsunaga, S., Kanou, K., Matsuzawa, A., Morishita, R., Kudoh, A., Shindo, K., Yokoyama, M., Sato, H., Kimura, H., Tamura, T., Yamamoto, N., Ichijo, H., Takaori-Kondo, A., and Ryom A. ASK1 restores the antiviral activity of APOBEC3G by disrupting HIV-1 Vif-mediated counteraction.
Nat. Commun., 6, 6945 (2015).

Review article

Kamiyama, M., Naguro, I., Ichijo, H. In vivo gene manipulation reveals the impact of stress-responsive MAPK pathways on tumor progression.
Cancer Sci., (review article), in press.

Sekine, S. and Ichijo, H. Mitochondrial proteolysis: Its emerging roles in stress responses.
B.B.A. (review article),1850, 274-280 (2015). 

Galluzzi, L. et al. Essential versus accessory aspects of cell death: recommendations of the NCCD 2015
Cell Death Differ., (review article), 22, 58-73 (2015).

Kawarazaki, Y., Ichijo, H. and Naguro, I. Apoptosis Signal-Regulating Kinase 1 (ASK1) as a therapeutic target.
Expert Opin. Ther. Tar., (review article), 18, 651-664 (2014).


Pulido R, Baker SJ, Barata JT,Carracedo A, Cid VJ, Chin-Sang ID, Dave V, den Hertog J, Devreotes P,Eickholt BJ, Eng C, Furnari FB, Georgescu MM, Gericke A, Hopkins B, Jiang X, Lee SR, Losche M, Malaney P, Matias-Guiu X, Molina M, Pandolfi PP, Parsons R, Pinton P, Rivas C, Rocha RM, Rodriguez MS, Ross AH, Serrano M, Stambolic V, Stiles B, Suzuki A, Tan SS, Tonks NK, Trotman LC, Wolff N, Woscholski R, Wu H, Leslie NR
A Unified Nomenclature and Amino Acid Numbering for Human
PTEN SCIENCESIGNALING 7(332) Pe15, 1-4, 2014

Maehama T, Kawahara K, Nishio M, Suzuki A, Hanada K
Nucleolar Stress Induces Ubiquitination-Independent Proteasomal Degradation of PICT1

Okamura K, Takayama K, Kawahara K, Harada T, Nishio M, Otsubo K, Ichijo K, Kohno M, Iwama E, Fujii A, Ota K, Koga T, Okamoto T, Suzuki A, Nakanishi Y
PICT1 expression is a good prognostic factor in non-small cell lung cancer
ONCOSCIENCE 1 (5), 375, 2014

Kajimoto H, Kai H, Aoki H, Uchiwa H, Aoki Y, Yasuoka S, Anegawa T, Mishina Y, Suzuki A, Fukumoto Y, Imaizumi T
BMP type I Receptor Inhibition Attenuates Endothelial Dysfunction in Mice with Chronic Kidney Disease

Wang L, Opland D, Tsai S, Luk CT, Schroer SA, Allison MB, Elia AJ, Furlonger C, Suzuki A, Paige CJ, Mak TW, Winer DA, Myers MG Jr, Woo M.
Pten deletion in RIP-Cre neurons protects against type 2 diabetes by activating the anti-inflammatory reflex
NATURE MEDICINE in press, 2014

鈴木班 研究分担者(仁科)

Sean Porazinski, Huijia Wang, Yoichi Asaoka, Martin Behrndt, Tatsuo Miyamoto, Hitoshi Morita, Shoji Hata, Takashi Sasaki, S.F. Gabby Krens, Yumi Osada, Satoshi Asaka, Akihiro Momoi, Sarah Linton, Joel B. Miesfeld, Brian A. Link, Takeshi Senga, Nobuyoshi Shimizu, Hideaki Nagase, Shinya Matsuura, Stefan Bagby, Hisato Kondoh, Hiroshi Nishina*, Carl-Philipp Heisenberg* and Makoto Furutani-Seiki* (2015)
YAP is essential for tissue tension to ensure vertebrate 3D body shape.
Nature 521, 217-221 (*Corresponding authors)

Yuta Motimaru, Morio Azuma, Natsuki Oshima, Yuta Ichijo, Kazuhiro Satou, Kouhei Matsuda, Yoichi Asaoka, Hiroshi Nishina, Takashi Nakakura, Chihiro Mogi, Koichi Sato, Fumikazu Okajima (2015)
Extracellular acidification activates ovarian cancer G-protein-coupled receptor 1 and GPR4 homologs of zebrafish.
Biochem. Biophys. Res. Commun. in press.

Yoichi Asaoka, Shoji Hata, Misako Namae, Makoto Furutani-Seiki and Hiroshi Nishina (2014)
The Hippo pathway controls a switch between retinal progenitor cell proliferation and photoreceptor cell differentiation in zebrafish.
PLoS ONE 9, e97365

Tadanori Shimomura, Norio Miyamura, Shoji Hata, Ryota Miura, Jun Hirayama and Hiroshi Nishina (2014)
The PDZ-binding motif of Yes-associated protein is required for its co-activation of TEAD-mediated CTGF transcription and oncogenic cell transforming activity.
Biochem. Biophys. Res. Commun. 443, 917-923.

Zeyu Yang, Kentaro Nakagawa, Aradhan Sarkar, Junichi, Maruyama, Hiroaki Iwasa, Yijun Bao, Mari Ishigami-Yuasa, Shigeru Ito, Hiroyuki Kagechika, Shoji Hata, Hiroshi Nishina, Shinya Abe, Masanobu Kitagawa, Yutaka Hata (2014)
Screening with a novel cell-based assay for TAZ activators identifies a compound that enhances myogenesis in C2C12 cells and facilitates muscle repair in the muscle injury model.
Mol. Cell. Biol. 34, 1607-1621.

Keita Nakanaga, kotaro Hama, Kuniyuki Kano, Takanoa Sato, Hiroshi Yukiura, Asuka Inoue, Daisuke Saigusa, Hidetoshi Tokuyama, Yoshihisa Tomioka, Hiroshi Nishina, Atsuo Kawahara and Junken Aoki (2014)
Overexpression of autotaxin, a lysophosphatidic acid-producing enzyme, enhances cardia bifida induced by hypo-sphingosine-1- phosphate signaling in zebrafish embryo.
J. Biochem. 155, 235-241.

Shuji Terai, Taro Takami, Naoki Yamamoto, Koichi Fujisawa, Tsuyoshi Ishikawa, Yohei Urata, Haruko Tanimoto, Takuya Iwamoto, Yuko Mizunaga, Takashi Matsuda, Takashi Oono, Miho Marumoto, Guzel Burganova, Luiz Fernando Quintanilha, Isao Hidaka, Yoshio Marumoto, Issei Saeki, Koichi Uchida, Takahiro Yamasaki, Kenji Tani, Yasuho Taura, Yasuhiko Fujii, Hiroshi Nishina, Kiwamu Okita, and Isao Sakaida (2014)
[review] Status and prospects of liver cirrhosis treatment by using bone marrow-derived cells and mesenchymal cells..
Tissue Engineering Part B: Reviews 20, 1-5.


Shimizu N, Ishitani S, Sato A, Shibuya H, Ishitani T.
Hipk2 and PP1c cooperate to maintain Dvl protein levels required for Wnt signal transduction.
Cell Rep. 2014 Sep 11;8(5):1391-404. doi: 10.1016/j.celrep.2014.07.040.
PMID: 25159144

Sa JK, Yoon Y, Kim M, Kim Y, Cho HJ, Lee JK, Kim GS, Han S, Kim WJ, Shin YJ, Joo KM, Paddison PJ, Ishitani T, Lee J, Nam DH.
In vivo RNAi screen identifies NLK as a negative regulator of mesenchymal activity in Glioblastoma.
Oncotarget. 2015 [Epub ahead of print]
PMID: 26023737

石谷班 研究分担者(岡田)

Kakumoto K, Ikeda J, Okada M, Morii E, Oneyama C.
mLST8 Promotes mTOR-Mediated Tumor Progression.
PLoS One. 2015 Apr 23;10(4):e0119015. doi: 10.1371/journal.pone.0119015. eCollection 2015.
PMID: 25906254

Oneyama C, Yoshikawa Y, Ninomiya Y, Iino T, Tsukita S, Okada M.
Fer tyrosine kinase oligomer mediates and amplifies Src-induced tumor progression.
Oncogene. 2015 Apr 13. doi: 10.1038/onc.2015.110.
PMID: 25867068